Summary of data analysis.

*The bottleneck for each biological replicate is calculated by determining the number of insertion sites lost from the input to the output in a set of known neutral genes. The median bottleneck is reported.
#The coverage specifies the total number of genes for which fitness values were obtained as a percentage of the total number of non-essential genes in the V. cholerae genome.
&A one-sample student's t-test with Bonferroni correction on genes with ≥5 insertion sites was used to determine if the fitness value is statistically different than 1.0.
##A student t-test with Bonferroni correction on genes statistically different than 1.0 was used to determine if the fitness value obtained in the test condition is statistically different that the fitness value obtained in the LB grown culture. Additionally, we required at least a 0.15 fitness difference from LB and a fitness value in LB of at least 0.82.