16S-V4 rRNA sequencing data from surface water, pore water, and groundwater collected from kettle-hole lakes, Cape Cod, MA (2015 - 2018). Cape Code Environmental Samples 2015-2018

Surface water, pore water, and groundwater were sampled within and downgradient of five groundwater flow-through ponds in Cape Cod, Massachusetts during different seasons from 2015 - 2018. The sampled ponds included Ashumet, Santuit, Snake, Shubael, and Longs whose condition ranged from oligotrophic to eutrophic. Pore water was collected between 15 to 100 cm below the pond bottoms on the upgradient and downgradient sides of the ponds. Groundwater was collected on the downgradient side where the pond water recharges the groundwater resulting in a pond shadow. Within the pond shadow, groundwater is under the direct influence of surface water. The pond shadows were sampled vertically and at various distances from their respective surface water sources. This set of data provides the original 16S-rRNA sequencing data derived from this set of water samples.