Dataset of Axonal Synapses, Acquired using a Two-photon Microscope in the Live Mouse Cortex

This Dataset consists of TIFF 100 images, split in 20 test and 80 training images, of axons with their synapses (boutons) labelled. The labels are in form of ground-truth binary images of the same size, in which the corresponding synapses have been labelled as boxes.

This data was collected in the live mouse cortex, using a two-photon microscope, with a 40x objective, at zoom 4, with a resolution of 512 x 512 x 0.147 microns per pixel, and a Point Spread Function characterised by a Full Width at Half Maximum (FWHM) values of 0.45 x 0.45 x 2.5 microns (x, y, z). 

Please cite the following paper when using this dataset:

Bass C, Helkkula P, De Paola V, Clopath C, Bharath AA. Detection of axonal synapses in 3D two-photon images. Giniger E, ed. PLoS ONE. 2017;12(9):e0183309. doi:10.1371/journal.pone.0183309.