Data from: Cell-free recombinase-integrated Boolean output system

Cell-free gene expression systems are increasingly important in
fundamental research and biomanufacturing, offering a versatile platform
for studying gene circuits and biocomputation. We present the cell-free
recombinase-integrated Boolean output system (CRIBOS), a site-specific
recombinase-based multiplex genetic circuit platform designed for
cell-free environments. With CRIBOS, we built over 20
multi-input-multi-output circuits, including 2-input-2-output genetic
circuits and a 2-input-4-output decoder. Combined with allosteric
transcription factor (aTF)-based sensors, the circuits demonstrate
multiplex environmental sensing. Moreover, utilizing paper-based CRIBOS,
which demonstrates remarkable portability and stability, we present a
biological memory storage logic circuit device that can preserve DNA-based
biological information for over 4 months with minimal resources, energy
costs, and maintenance requirements. Implementing CRIBOS not only expands
the application of multiplex Boolean logic gates from cellular systems to
the cell-free environment but also augments their overall versatility,
opening new avenues for designing and applying sophisticated genetic
circuits.