Shotgun Metagenomic Analysis of the Tumor and Non-Tumor Regions in Bladder Cancer

This study used shotgun metagenomic sequencing to compare microbial communities in paired tumor and non-tumor tissues from 35 bladder cancer patients. Results showed distinct microbial profiles, with reduced diversity and dominance of specific taxa in tumor regions. Escherichia coli and Helicobacter pylori were enriched in non-tumor tissues and demonstrated anti-proliferative effects in vitro, suggesting potential protective roles. The findings highlight novel microbial biomarkers and provide insights for microbiome-based strategies in bladder cancer.