Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line in medium with 100% and medium with 25% of amino acids. [aa]

Mutant p53 can acquires oncogenic properties supporting tumor growth, metastases and chemoresistance, by reprogramming cancer cell transcriptome, proteome and metabolome. To investigate what is the gene expression network regulated by mutant p53 in condition of limited amino acids availability, we silenced mutant p53 expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line, grown in medium with 100% and medium with 25% of amino acids. We performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MDA-MB 231 cells, grown in medium with 100% and medium with 25% of amino acids and transfected with Control or p53 siRNAs, each in duplicate.