Sequencing of siRNA from soybean containing silencing vectors and empty vectors

A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed Examination of siRNA corresponding to Ss-oah1 in S. sclerotiorum in plants containing silencing constructs and plants containing empty vectors