Host response to Leishmania amazonensis and OPN role in immune responses. In vitro.

Transcript profiles encoding for IL-1β and for iNOS, STAT-1, IL-17 (Th1) and IL-10, IL-4 (Th2) cytokines in BMF isolated from NOD+/+ (WT) and NOD.opn-/- (KO) mice in response to L. amazonensis. Cells were inoculated with Leishmania amazonensis amastigotes at a MOI: 4:1 (parasites: macrophages) and incubated for 24h and 48h before lysis for RNA preparation. Mean fold changes for transcripts and standard deviations have been calculated by using as a calibrator the wild-type non-infected value at each time point.