Estimated sensitivity and specificity of panels and markers for detection of MMR-deficient lesions.

The MMR status of 90 lesions was determined by IHC for MLH1, MSH2, PMS2 and MSH6. MSI data was not available in all lesions; therefore the result categories do not sum to 90. There were 12 lesions that did not stain for one or more MMR proteins and these were considered true positives for loss of MMR. There were 78 lesions that stained normally for all MMR proteins and were considered true negatives with normal MMR expression. For panels, a result of MSI-High was considered positive, and a result of MSI-Low or MSI stable was considered negative. The difference in sensitivity between the LMR panel and the both the MSI Analysis System and the NCI panel was statistically significant (z-test; p<0.001). The specificity of the LMR panel was not significantly different from the MSI Analysis System or the NCI panel (z-test; p = 0.252 and 0.899, respectively).
Estimated sensitivity and specificity of panels and markers for detection of MMR-deficient lesions.