Overview of identified heat shock proteins via LC-MS/MS.
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HEK293T cells were transfected with Flag-hGRα via calcium phosphate and following stimulation with either solvent (NI) or 10μM CpdA (CpdA), immunoprecipitated using Flag beads (plasmid and methodology described in [7]. Two identical set-ups were done in parallel, yet, using two different elution methods, via NH4OH, as indicated, or via the Flag peptide (FLAG, 100μg/ml). The Swiss-Prot accession is indicated in the protein description field. Per protein, the number of identified MS/MS spectra (at 99% confidence settings (Mascot)) is indicated.
创建时间：
2015-12-02
