Circular dichroism spectra of sTMEM2 incubated overnight at 4°C and 37°C. sTMEM2 at a concentration of 0.12 mg/ml in 50 mM phosphate buffer, pH 6.0 was incubated overnight at 4°C and 37°C. Spectra wer
aThe Thiosulfate∶cyanide sulfurtransferase activity of MBP-MoeZR and MBP-MoeBR were measured by the colorimetric assay after Sörbo [21]. KM and kcat values were determined by varying concentrations of