SPP1+ Macrophages Contribute to Hair Growth in Contact Hypersensitivity [spatial transcriptomics]

Using Visium HD spatial transcriptomics and immunostaining, we mapped skin compartment dynamics during DPCP-induced allergic contact dermatitis. Dermal white adipose tissue transitioned from metabolic homeostasis to immune activation and inflammatory remodeling, accompanied by sustained lipolysis. Concurrently, CD45? immune cells accumulated in the lower dermis and dWAT. These findings identify dWAT as an immune-metabolic hub orchestrating inflammatory progression and highlight spatial transcriptomics as a powerful tool for dissecting skin niche regulation. Overall design: Spatial transcriptomics was conducted using the Visium HD (10X Genomics) system. Skin tissue was collected on days 1, 2, and 3 after DPCP challenge and from the control group on day 0. All samples were embedded in one paraffin block.