Host Acid Signal Controls Salmonella Flagella Biogenesis through CadC-YdiV Axis

Upon entering host cells, Salmonella quickly turn off flagella biogenesis to avoid recognition by the host immune system. However, it is not clear which host signal(s) Salmonella senses to initiate flagellum control.During the infection of a host,acid pH acts as an important environmental factor to triggers some genes expression. To investigate the effect of environmental acid signaling on the regulation of Salmonella flagella synthesis, we cultivated Salmonella typhimurium ATCC 14028s to an OD600 of 0.4, and then continued to culture for 2 h at pH 7.0 or pH 5.0. We then performed gene expression profiling analysis using data obtained from RNA-seq of cultured cells at different pH condition. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Salmonella typhimurium ATCC 14028s under differnent pH condition (pH 7.0 or pH 5.0),and each set has 3 repetitions .