Transplanting FVIII/ET3-Secreting Cells in Fetal Sheep Increases FVIII Levels Long-Term Without Inducing Immunity or Toxicity

To investigate the differentiative fate of human PLCs following transplantation into fetal sheep and engraftment in various tissues/organs, we performed gene expression profiling analysis using data obtained from RNA-seq of human PLCs prior to in utero transplantation and of each engrafted fetal sheep tissue after filtering to remove any cross-reactivity with orthologous sheep transcripts Overall design: Comparative gene expression profiling analysis of RNA-seq data for human placental cells (PLCs) prior to and following engraftment within various sheep tissues after in utero transplantation