Comparative wheat phosphoproteome profiling pinpoints high temperature-associated breeding markers

Elevated temperatures resulting from climate change are adversely impacting natural and crop ecosystems, necessitating the development of heat-tolerant crops. We established a framework to precisely identify wheat protein-phosphorylated sites associated with varying temperature sensitivities. Our findings demonstrate that the phosphorylation state of a specific set of proteins creates a unique signature for heat stress tolerance. These findings aid the identification of targets for breeding or genome editing to enhance heat tolerance.