Increased level of glutathione contributes to stress tolerances and global translational changes in Arabidopsis

Polysomal profiling results showed that global translation was enhanced in roots after 8 h and in shoots after 24 h of GSH treatment. By performing transcriptomic analyses of steady-state and polysome-bound mRNAs in GSH-treated plants, we reveal that GSH has more a greatersubstantial impact on translational change. GSH-induced gene expression in Arabidopsis roots and shoots was measured seperately at 8 and 24 hours after exposure to 100 μM GSH. Two independent experiments were performed at each time (8 or 24 hours).