Comparative metabolome and transcriptome analysis of fragrant rice unveils the correlated metabolites and transcripts with 2-acetyl-1-pyrroline (2AP)

Here we studied comparative metabolome and transcriptome between a fragrant variety and a non-fragrant variety in four development stages. Our study provides comprehensive insights into the genetic and metabolic basis of fragrance in the SSXN variety of rice. Through a combination of metabolome and transcriptome analyses, coupled with WGCNA and correlation analyses, we elucidated key pathways and molecular components associated with fragrance metabolites production. Our findings not only underscore the pivotal role of mutations in the BADH2 gene, which result in the accumulation of 2AP, but also shed light on the involvement of other metabolites and transcripts contributing to the fragrance of rice grains. Additionally, transcriptome analysis revealed differential expression patterns of genes related to fragrance metabolites, further enhancing our understanding of the regulatory mechanisms underlying aroma development in rice.