Anthrax toxins regulate pain signaling and can deliver molecular cargoes to Antxr2+ DRG sensory neurons

We found that intrathecal injection of anthrax edema toxin (ET), causes analgesia in adult B6J mice. In order to determine if DRG neuron transcriptional responses play a role in pain blockade, we performed a bulk RNA-seq experiment on dissected mouse DRG neurons. We report that anthrax edema toxin causes transcriptional responses in DRG neurons 2h after in vivo administration Overall design: We injected 6 mice (intrathecally) with PBS (control) and 6 mice with ET. After waiting 2h and observing pain blockade in the ET injected animals, we dissected the lumber DRGs and proceeded to RNA-extraction