TRIM33 binding in hematopoietic cell lines and effects of TRIM33 deletion on RNA PolII occupancy during activation of primary macrophages

To get insight into lineage-specific TRIM33 functions, TRIM33 ChIP-seq was carried out in non-differentiated erythroid (MEL NI), differentiated erythroid (MEL I), immature myeloid (32D). In addition, we compared the genome-wide profiles of RNA PolII occupancy between wild-type bone marrow-derived macrophages (BMDM) and Trim33-/- BMDM activated with LPS. Overall design: Chromatin immunoprecipitations of TRIM33 and RNA PolII followed by multiparallel sequencing performed in hematopoietic cell lines and murine bone marrow-derived macrophages (BMDM).