Genome-wide binding targets of GBF2 in ER stress

To profile in vivo DNA-binding activities of GBF2 in the UPR, we next perform chromatin immunoprecipitation sequencing (ChIP-seq) analyses of GBF2 at 0 h of ER stress recovery (i.e., 6 h of Tunicamycin, an ER stress inducer, treatment or DMSO) using a GBF2 native promoter-driven yellow fluorescent protein for energy transfer (YPet)-tagged GBF2 line (pGBF2:GBF2-YPet). Our analysis pipeline generated 523 Tm sample-specific binding peaks (i.e., not overlapped with peaks found in the corresponding DMSO-treated samples or overlapped with three times higher intensity) of GBF2. Hereafter we refer to these peaks as the UPR-specific binding peaks. A total of 8 samples, which consists of 1 protein with 2 treatments at 1 time-point in 2 biological replicates, were processed.