Gene expression profiling of adult C. elegans in response to heat hormesis regimen

To understand the molecular basis of heat hormesis, we adapted a regimen in which C. elegans that had been cultured at 20°C were exposed to 30°C for 6 hours during early adulthood (referred to as 'priming'), allowed to recover for 12 hours at 20°C, and then challenged with heat shock (HS) at 35°C. We applied this hormesis regimen to wild-type (N2) or germline-less glp-1(e2144) worms. Overall design: Gene expression profiling was conducted using 3' mRNA sequencing of Naïve (N) and Primed (P) groups in glp-1(ts) mutants or wild-type (N2) worms at Timepoints 1 (after Priming), 2 (Recovery), 3.3 (upon 3-hour HS), 3.6 (upon 6-hour HS), and NC (negative control-before priming) across the heat hormesis regimen. For the glp-1 mutant, five biological replicates were included for each condition, except for Timepoint 3.6, which had three biological replicates. For N2, there were two biological replicates per condition.