RAB GTPase proximity interactome

RAB GTPases represent the most abundant family of small GTPases and regulate multiple aspects of membrane trafficking events, from cargo sorting to vesicle budding, transport, docking, and fusion. To control these processes, RABs are tightly regulated by guanine exchange factors (GEFs) and GTPase-activating proteins (GAPs). Activated RABs recruit effector proteins to regulate trafficking events. Identifying RAB-associated proteins has proved difficult, as their association is often transient. Recent advances in proximity labeling approaches that allow for covalently labeling protein neighbors of proteins of interest now permit cataloging proteins in the vicinity of RAB GTPases. Here, we provide APEX2 proximity labeling on 23 human RABs and their neighboring proteomes.