Differential transcriptome responses in human THP-1 macrophages following exposure to T98G and LN-18 human glioblastoma secretions: a simplified bioinformatics approach to understanding patient-glioma-specific effects on tumor-associated macrophages

We first describe a methods approach to differentiating THP-1 monocytes to macrophages with M0-like genotype. Then we describe the effect that different glioblastoma supernatants have on the gene expression profiles of macrophages, suggesting gliomas should be considered different diseases. Differential gene expression analysis of RNA-seq data for THP-1 monocytes, differentiated macrophages, and those same macrophages treated with different glioma supernatants.