Transcriptome changes caused by RBP-L knockdown in rice grains

A T-DNA insertion within RBP-L 3’UTR resulted in 10-25% expression level of RBP-L gene compared to wild-type. The reduced expression of RBP-L caused partial mis-localization of glutelin and prolamine RNAs and conferred other general growth defects including dwarfism, late flowering and smaller seeds. Transcriptome analysis showed that RBP-L knockdown greatly affected the expression of prolamine family genes and many genes invovled in essential biological pathways during plant development. Total 6 samples were anlyzed, including 3 biological repeats from wild type (ZH11) and RBP-L knockdown (rbpl) line.