iTRAQ-based Quantitative Proteomics Analysis of protein profile response to Pink1 deletion in breast cancer

ITRAQ technique was applied to compare the comprehensive protein expression profiles between MDA-MB-231con and MDA-MB-231Pink1-/- cells, and the original mass spectrum data were searched using Mascot engine against the UniProt database and the decoy database. Proteins were identified to be significantly according to the cut-off of a fold change ≥1.2 or ≤0.83 and a P-value ＜0.05. All of the pathways and GO enrichment analyses were performed to identify the critical proteins induced by Pink1 deletion.