Genome wide expression analysis of young and aged human endometrium

We use RNA-sequencing to measure global transcription in LH timed endometrial biopsies of 10 women aged = 30 years and 10 women aged = 40 years. Overall design: 10 endometrial biopsies were taken from 10 women aged = 30 years, and 10 women aged = 40 years. The 2 groups were matched for BMI, previous live births, and previous 1st or 2nd trimester losses. Biopsies were LH surge timed and were taken at between LH +6 to LH +10. Biospies were snap frozen before RNA extraction and subsequent RNA library preparation.