Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages

We developed ONT-cappable-seq, a specialized long-read RNA sequencing technique that allows end-to-end sequencing of primary prokaryotic transcripts using the Nanopore sequencing platform. We applied ONT-cappable-seq to study the transcriptional landscape of Pseudomonas aeruginosa phage LUZ7, leading to a comprehensive genome-wide map of viral transcription start sites, terminators and complex operon structures that fine-regulate gene expression. At the same time, it provides new insights in the RNA biology of LUZ7 and paves the way for more in depth transcription studies that can help unveil the complex layers of phage-host interactions. Full-length transcriptional profiling of P. aeruginosa US449 infected with phage LUZ7 at different time points (0, 5, 10 and 20 minutes) using ONT-cappable-seq.