RNA-seq analysis of microglia and infiltrating macrophages in the injured retina. Mus musculus

The goal of this study is to identify genes differentially expressed between microglia and infiltrating macrophages in the injured retina Methods: mRNA profiles of microglia (Cont) and infiltrating macrophages (GFP) at day 7 post-injry, quadruplicated, were generated by deep-sequencing using Illumina HiSeq 2500 sequencer. Results: RNA-seq tags were mapped to the mouse mm9 reference genome assembly using Tophat2 ver. 2.0.8b, with FPKM values caluculated with Cufflinks ver. 2.1.1 with the default option.Comparison of gene expression between microglia and infiltrating macrophages identified genes specific to each subset. Conclusions: Our study provides the detailed analysis of transcriptomes in microglia and infiltrating macrophages in the injured retina Overall design: mRNA profiles of microglia (Cont) and infiltrating macrophages (GFP) harvested from the DTA-injured retina at day 7 post-injury were generated by deep-sequencing using Illumina HiSeq 2500.