Optimization of transposon-mediated gene transfer conditions in mice.

Figshare2023-06-09 更新2026-04-28 收录

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Optimization of transposon-mediated gene transfer conditions in mice.

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2023-06-09

Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering

基因组测序技术优化

基因工程

The Cas9 endonuclease is used for genome editing applications in diverse eukaryotic species. A high frequency of off-target activity has been reported in many cell types, limiting its applications to

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Revised Selection Criteria for Candidate Restriction Enzymes in Genome Walking

基因组测序技术优化

限制性内切酶筛选策略

A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus sever

NIAID Data Ecosystem50

Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube

基因组测序技术优化

药物代谢基因

Genotyping of thiopurine S-methyltransferase (TPMT) is recommended for predicting the adverse drug response of thiopurines. In the current study, a novel version of allele-specific PCR (AS-PCR), terme

Figshare2016-01-18 更新20

Application of a multiplexed ligase end-joining fidelity assay to optimize Golden Gate assembly

基因组测序技术优化

连接酶保真度分析

Using a comprehensive method for profiling end-joining ligation fidelity and bias to predict highly accurate sets of connections for ligation-based DNA assembly methods

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Table_3_Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes.DOCX

CRISPR-Cas分子机制

基因组测序技术优化

CRISPR-Cas systems employ ribonucleoprotein complexes to identify nucleic acid targets with complementarity to bound CRISPR RNAs. Analyses of the high diversification of these effector complexes sugge

NIAID Data Ecosystem50

Optimization of transposon-mediated gene transfer conditions in mice.

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