Phe-restriction_A375

To optimize the efficacy of boron neutron capture therapy (BNCT), we investigated the optimal conditions for maximizing the uptake of boronophenylalanine (BPA), an analog of L-phenylalanine (L-Phe), in tumor cells. In the cultivation of tumor cell lines, some cells showed an amplified accumulation of BPA under 24 hours of L-Phe deprivation, which resulted in an enhancement of BNCT efficiency. In contrast, A375 demonstrated unaltered responsiveness in the uptake of BPA under L-Phe restriction. Accordingly, we conducted a comprehensive analysis of the cellular response of A375 cells under L-Phe restriction by RNA-seq.