Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17062
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To identify factors involved in glioma-initiating cells (GICs), we compared gene expression between GIC-like cells and non-GICs. Neural stem cells (NSCs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s) were purified by flow cytometry. Total RNA was prepared using RNeasy Mini Kit (QIAGEN).
创建时间：
2019-02-11
