Primary colonization of hydroxyapatite by canine oral bacterial isolates.

Quantity (Crystal violet) and respiratory activity (Alamar blue) measured after 2 hours interaction with the surface followed by 24 hours growth.Adhered bacteria stain dark purple with Crystal violet. The Alamar blue stain becomes pink in the presence of respiratory activity. Relative intensity of color change measured visually and ranked on a four point scale from most to least intense (+++, ++, +, -). Each score is the result of analysis of three biological replicates.Primary colonization of hydroxyapatite by canine oral bacterial isolates.