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RT-qPCR quality control (R2, M and Efficiency) for each reaction.

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Figshare2015-12-02 更新2026-04-29 收录
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1. WT mice from R126, C57BL/6j and C57BL/10 mouse strains, 2. R129 WT and Actn3 KO mice, 3. C57BL/6j WT and Actn3 KO mice, 4. C57BL/10 WT and Actn3 KO mice for each selected gene. R2 = correlation coefficient (optimal R2 = 1), M = the expected average Cq (quantification cycle number) between each 10-fold standard curve dilution (optimal M = –3.3) and E = the RT-qPCR reaction efficiency (optimal E = 1.00).

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2015-12-02
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