Role of HO-1 in the antioxidant and renal protective effect of EGCG in CIN.

EGCG (10mg/kg body wt) was intravenously infused 15min before the establishment of CIN. The HO-1 inhibitor ZnPP (30mg/kg body wt) was injected intraperitoneally 7h before EGCG pretreatment. The rats were sacrificed at 24h after the establishment of CIN. Renal MDA level (A) and SOD activity (B) were measured. Renal HO-1 protein detected by immunoblotting was expressed as the ratio of HO-1 and GAPDH (C). Serum Cr (D), and BUN (E) were determined to evalute the renal function. Nrf2 expression in the nuclear fraction of renal tissue detected by immunoblotting was expressed as the ratio of Nrf2 and histone H3 (F). (n = 5, * P<0.05 vs. vehicle; # P<0.05 vs. CM; & P<0.05 vs. EGCG).