A single cell altas of mouse embryonic diapause and peri-implantation development

Here, we molecularly defined embryonic diapause at single-cell resolution, revealing hidden transcriptional dynamics while seemingly the embryo resides in a state of suspended animation. Alongside, we found that the dormant pluripotent cells are “aware” of their microenvironment which sustains their viability via Yap-mediated mechanotransduction. Overall design: Approximately 200~250 blastocysts from each developmental stage – E4.5, EDG7.5, EDG9.5 and Reactivated and 50 embryos from E5.5 stage were collected and analyzed using sc-RNA seq.