10X Genonics Drop-seq single-cell RNA-seq of neonatal (P1.5) testis cells from mice treated with DMSO or Retinoic Acid.

To reveal distinct transcriptomes associated with response of neonatal prospermatogonia to retinoic acid (RA), unselected testis cells from animals treated with RA or DMSO were used for Drop-Seq analysis 12 hours after treatment at P1. Unselected testis cells isolated from animals 12hr after treatment with DMSO or RA at midnight of P1 were used for this study. Two replicate experiments were performed using testes pooled from separate litters. Data are from a total of 34,153 cells. The data from these experiments are stored in Loupe Cell Browser files (.cloupe) which are generated during analysis of 10X Genomics Single-cell data and can be opened and queried with the Loupe Cell Browser (10X Genomics). This software can be downloaded for free from https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest. It is important to note that the companion manuscript for these data used additional analyses that are not represented in these files.