RNA-Seq to investigate gene expression in M. bovis BCG subjected to darG depletion or DNA damage induction with mitomycin D

M. bovis BCG bacilli were subjected to silencing of the darG antitoxin gene by CRISPRI, leading to unregulated activity of the toxin DarT. This results in ADP-ribosylation of specific motifs in genomic DNA, and induction of the DNA damage response. The goal of this study is to compare gene expression of M. bovis BCG in untreated, DarG-depleted, and mitomycin treated samples. Comparison of gene expression of M. bovis BCG in untreated, darG antitoxin depleted, and mitomycin C treated samples