Geographical origin and in vitro features of Peruvian L. braziliensis isolates included in the study.

SbV and SbIII: in vitro susceptibility to antimony (pentavalent and trivalent, respectively), expressed as an ‘activity index’ (A.I.), i.e. as the ratio of the ED50 (50% effective dose) of that tested isolate to the ED50 of the WHO reference L. braziliensis strain MHOM/BR/75/M2903. Isolates with an A.I. of 0–2 were considered sensitive to the tested drug (0, more sensitive than the reference strain M2903), while isolates with an A.I. of 3 or higher were considered resistant. Data shown were reported in Yardley et al. [8]. Tx outcome: clinical treatment outcome at the 12-month follow-up of respective patients. Prom (slope): the slope of the promastigote growth curve was calculated from the equation of the best-fitted line, considering all time points until maximum density was reached (from duplicate experiments). Prom (max density): maximal density achieved during stationary phase; average of the densities at the time when maximal density was reached and 24 hours later (from duplicate experiments). % inf. Mf: percentage of infected macrophages at 24 hours post-infection. N. ama/Mf: average number of amastigotes per macrophage at 24 hours post-infection; all amastigote experiments were done in duplicate. nd: not done.