Differential expression of human BM-MSCs RNA-Seq and 21-day of chondrogenic differentiation from high-depth transcriptomic profiling of mesenchymal stem cells during chondrogenesis

A comprehensive evaluation of differential gene expression on RNA-seq data of early and late passage hMSCs from three individuals, and undifferentiated and 21-day of chondrogenic differentiation hMSCs from previous publication (GSE109503). Genome-wide RNA-seq and differential expression analysis revealed that signalings such as Pulmonary Fibrosis Idiopathic (IPF) Signaling Pathway and Osteoclasts in Rheumatoid Arthritis (RA) Signaling Pathway were significant differences in early MSC. Differential FOXO1 expression, higher in Early than Late MSCs, was responsible for promoting suspension survival, and cell migration, and induces chondrogenic differentiation. Overall design: RNA-Seq of early and late passage bone marrow-derived mesenchymal stem cells with 3 biological replicates (representing 6 samples). In addition, third-party reanalysis of 6 samples from Series GSE109503.