Spodoptera frugiperda Sf-RVN cell line Genome and Transcriptome sequencing and assembly

We recently isolated Sf-RVN, a new Spodoptera frugiperda (Sf) cell line that unlike other Sf cell lines is not persistently infected with Sf-rhabdovirus. Here, we used massively parallel sequencing (MPS) to more globally examine Sf-RVN for adventitious viral contaminants. We found no evidence of contamination with Sf-rhabdovirus or any other virus. Thus, this study documents the enhanced biosafety profile of Sf-RVN as compared to other Sf cell lines, and supports the notion that Sf-RVN is highly suitable for the production of safe biologicals.