Four cases of death from systemic immune response syndrome after COVID-19 vaccination

The present study examined the relationship between the causes of unknown death and COVID-19 vaccination. Investigated the gene expressions associated with the causes of death in unknown death cases. The samples were obtained from Hiroshima University autopsy cases. The mRNA was extraction from blood samples was performed using ISOGEN-LS. Transcriptome profiling using next-generation sequencing was conducte. Total RNA (400 ng) was treated using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) to eliminate rRNA and prepare the cDNA library. Synthesis of cDNA followed by transcriptome library preparation was conducted using the MGIEasy RNA Directional Library Prep Kit V2.0, in which dUTP were incorporated in second-strand cDNA synthesis instead of dTTP, to block PCR amplification against the second-strand templates and enable strand-specific transcriptome profiling. A 12-cycle PCR amplification was performed to increase library yield. The resulting transcriptome sequencing libraries were quantified by Qubit DNA Assay and their fragment size distribution was confirmed by TapeStation D1000 ScreenTape.