RNAseq analysis of cochlear glial sphere and induced neurons transcriptomes from SCDM, SCDM/FGF and FIBCL differentiation in vitro

To evaluate the maturity under different induction conditions of induced neurons, we performed transcriptome sequencing analysis. Pearson correlation analysis showed that the expression profiles of neurons induced by SCDM and SCDM/FGF are relatively close to that of spheres, while the overall expression of small molecules iNs is quite different from the above three types of cells, which is more similar with the transcriptome expression of primary SGN. These results generally show that small molecules effectively promote the neural differentiation towards SGN. Examination of the gene expression pattern and neuronal maturation of small molecules FIBCL induced neuron from cochlear glial sphere