Illumina and PacBio amplicon sequencing for Recursive Editing

Sequencing reads from short read (Illumina) and long read (PacBio) amplicon sequencing for Recursive Editing. Samples were amplified from genomic DNA from genome edited human cells. Short read sequencing reads were used to assess genome editing efficiency at the indicated loci. Long read sequencing reads (~6 kb) allowed quantification of large deletions arising from genome editing.