Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [Agilent]

Purpose: To understand how gene expression changes in yan and pnt mutant embryos A custom expression array was designed on the Agilent GE 8x15K platform. Probes were designed using the Earray software by Agilent to represent putative Yan target genes. RNA was extracted froms stage 11 wildtype, yan null or pnt null embryos with TRIzol following manufacturer's protocol and purified using an RNeasy Mini Kit from Qiagen. Total RNA was labeled and hybridized according to standard protocol using the Quick Amp One-Color Labeling Kit from Agilent. Triplicate experiments were performed for each genotype.