YAMAT-seq analysis of APOBEC3A-mediated tRNA deamination in vitro

This study contains YAMAT-seq data generated to investigate APOBEC3A-mediated tRNA editing in vitro. Mature tRNA fractions were isolated from HEK293T cells (Homo sapiens) and incubated with recombinant wild-type APOBEC3A, a catalytically inactive APOBEC3A mutant (E72A), or without enzyme as a control. Deamination reactions were performed in three technical replicates for each condition. Independent YAMAT-seq libraries were prepared from each replicate and sequenced to analyze C-to-U editing events in tRNA molecules. The dataset provides raw sequencing reads generated from in vitro-treated and control tRNA samples to enable analysis of APOBEC3A-mediated tRNA editing signatures.