Bactericidal agents resistance assay
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Overnight cultures of <i>Streptococcus gordonii</i> were harvested and resuspended in RPMI medium (pH 7.0), and then 5x10<sup>5</sup> cells/ml of bacteria were transferred to RPMI medium (pH 7.0 or 5.0) supplemented with lysozyme (FUJIFILM, Osaka, Japan) (5 mg/ml), defensin (PEPTIDE, Osaka, Japan) (5 microgram/ml), lactoferrin (Sigma-Aldrich) (10 microgram/ml), or a mixture of these bactericidal agents. After 2 h of incubation at 37 °C, samples were serially diluted and plated on BHI agar.
创建时间:
2021-11-25



