Data and code for: A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila (doi: https://doi.org/10.1101/2024.03.28.587162)

Raw data and R code for:

Tiina S. Salminen, Laura Vesala,  Yuliya Basikhina, Megan Kutzer, Tea Tuomela, Ryan Lucas, Katy Monteith, Arun Prakash, Tilman Tietz and Pedro F. Vale. A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila  (doi: https://doi.org/10.1101/2024.03.28.587162)

Data files:

CFU_stats.R: statistics for CFU data

Prettgeri_CFU.csv: colony forming units (CFUs) in P. rettgeri-infected flies

Saureus_CFU.csv: colony forming units (CFUs) in S. aureus-infected flies

gene exp_mitotypes-stats.R: statistics for the qPCR data

gene exp_mitotypes.csv: data on the relative gene expression of FucTC, AANATL3, Acp1 and CG3397 among mitotypes

copynumber_stats.R: statistics for the copynumber data

copynumber.csv: Mitochondrial copy number as copies of the mtDNA target gene 16S relative to nuclear target gene RpL32

respirometry_stats.R: statistics for the respirometry data

respirometry.csv: Mitochondrial respiration measured in uninfected male flies by measuring oxygen consumption of the OXPHOS complexes I, III and IV

ROS_stats.R: statistics for ROS

ROS.csv: hydrogen peroxide (H2O2) quantification (whole flies)

wasp_stats.R: Statistics on the proportion of melanised and unmelanised wasp larvae

Wasps_data.csv: Data on proportion of melanised (killed) and unmelanised (living) L. boulardi parasitoid wasp larvae found in Drosophila larvae

CC_stats.R: statistics on crystal cell counts

CC.csv: amount of hemocytes called crystal cell in the larvae

HC_stats.R: statistics on hemocyte counts

hc_uninf.csv: total and activated hemocyte (blood cell) counts in uninfected larvae

hc_inf.csv: total and activated hemocyte (blood cell) counts in infected larvae (48 h after infection by L. boulardi parasitoid wasps)

PPO3.R: statistics on PPO3 expression levels

PPO3.csv: data on gene expression level of prophenoloxidase (PPO3)

MMP.R: statistics on MMP

cybridMMP.csv: mitochondrial membrane potential (MMP) measured from larval hemocytes using the TMRM dye

CellROX.R: statistics on CellROXTM signal

CellROX.csv: Reactive oxygen species (ROS) measured from larval hemocytes using CellROXTM green reagent

Hazard.Rmd: hazard ratios

Combined.xlsx: data on hazard ratios Hazard ratios of survival post P. rettgeri, S. aureus, Kallithea and DCVinfections

Mito_priming.R: Statistics on the effect of priming

mito_priming.csv: data on the effect of immune priming with heat-killed bacteria prior to infection

hc_stats_supplementary.R: statistics for sex-specific differences in hemocyte counts (data: hc_uninf.csv & hc_inf.csv )

PI_hc_stats.R: statistics on dead (Propidium iodide, PI-positive) vs. living (PI-negative) larval hemocytes (data: hc_uninf.csv & hc_inf.csv )

melanisation_stats.R: statistics on melanisation response

melanisation_males.csv: melanisation response in hemolymph