Lethal Factor is measurable in the ear, draining cervical lymph nodes and serum at the time of debridement 12 hours after spore inoculation.

AInjection of 1×106 luminescent 7702 (BIG23) spores subcutaneously into left ear of 3 A/J mice.BInjection of 1×106 triple exotoxin knockout (TKO) spores with 100 ng LF plus 650 ng PA into left ear of 3 A/J mice.CLF detected by anatomic locations (pg/tissue ± SE) 12 hours post inoculation. Average of three separate experiments with three mice.DCervical lymph node.