TTV.xlsx

The viral metagenome was assessed in plasma samples from 240 TTV-PCR-positive healthy blood donors. TTV-specific reads were assembled into TTV-specific contiguous sequences (contigs) using 49,087 viral complete genomes obtained from the NCBI GenBank nuccore database. The number of functional-inhibitory peptides was then assessed. TTV ORF1-specific contiguous sequences were therefore mapped to published TTV sequences using the ICTV threshold of 69% nucleotide identity of the complete ORF1 to annotate the corresponding TTV strains. The number of inhibitory peptides was then assessed in the published TTV strains and reassigned to the TTV strains found in the blood donors.