Diagnosis of Kawasaki Disease in children using host RNA expression [Discovery_Dataset]

Genome-wide analysis of transcriptional profiles in children <17 years of age with inflammatory diseases, bacterial or viral infections or with clinical features suggestive of infection. Patients were recruited at or after presentation to hospital. Main inclusion criteria were presence of illness of sufficient severity to warrant blood tests for clinical reasons, in child aged <17 years. Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 V4 Beadchips (Discovery set). Numbers of arrays analysed in the discovery set were as follows: Kawasaki Disease (n=78), Healthy controls (n=55), Definite Bacterial infection (n=52), Definite Viral infection (n=94), Inflammatory diseases (n=84), infections of uncertain bacterial or viral aetiology (n=96).